Cathepsin K Substrate II; Fluorogenic

General description

An internally quenched, highly cathepsin K-selective, fluorogenic peptide substrate that is efficiently cleaved by CatK (k_cat/Km = 0.426 µM_-¹ S_-¹), but not by Cathepsins B/L/S/F/H/V.

An internally quenched, highly cathepsin K-selective, fluorogenic peptide substrate that is efficiently cleaved by CatK (k_cat/K_m = 0.426 µM^-1 S^-1), but not by Cathepsins B/L/S/F/H/V. Studies using WT and CatK^-/- mice reveal that the substrate is not cleaved by other proteolytic enzymes present in fibroblast lysates.

An internally quenched, highly cathepsin K-selective, fluorogenic peptide substrate that is efficiently cleaved by CatK (k_cat/K_m = 0.426 µM^-1 S^-1), but not by Cathepsins B/L/S/F/H/V. Studies using WT and CatK^-/- mice reveal that the substrate is not cleaved by other proteolytic enzymes present in fibroblast lysates. Emission Max: 420 NM, Excitation Max: 320 nM.r>

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Lecaille, F., et al. 2003. Biochem. J.375, 307.

Packaging

1 mg in Plastic ampoule

Packaged under inert gas

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Sequence

Abz-His-Pro-Gly~Gly-Pro-Gln-EDDnp

Warning

Toxicity: Standard Handling (A)